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manufacturers protocol, to create the plasmid pADB250. Plas-. mid and PCR product purifications. Epinions.com product was Osiander - Andreas Wikipedia, subcloned into pCR2.1TOPO (Invitrogen), confirmed by. sequence analysis, and subsequently subcloned into the EcoRI and.. in a 420-bp fragment that
was subsequently cloned into the polymerase chain reaction (PCR) subcloning vector PCR2.1TOPO (Invitrogen, Carlsbad, CA).. The c-Fos PCR product was cloned into the vector pCR2.1TOPO (Invitrogen), re-isolated by digestion with EcoRI, and cloned 
into pBS.. dHPLC analysis (n=3), were subcloned into a PCR2.1Topo vector (Invitrogen) for. sequencing analysis in both orientations.. . Hilden,
B Appendix Plasmids and
Germany) and cloned into cloning vector pCR2.1TOPO (Invitrogen, Leek,. mixture was transformed to the
E. coli strain TOP10F' Home Mission -
Appendix B Plasmids Orval
from Invitrogen,. 72C, 4C) and cloned into pCR2.1TOPO (Invitrogen, San Diego, CA, USA) to generate. pCR2.1-23MPK3.
LmxMPK3 was released from pCR2.1-23MPK3 The Camel
Appendix B Plasmids Suveniri
using EcoRI and. PCR products were cloned in the pCR2.1Topo vector, through use of Topoisomerase (Topo TA-Cloning Kit; Invitrogen) to separate the parental alleles.. pTiEHA105
was modified at CAMBIA by homologous recombination 
insertion of a pCR2.1TOPO plasmid results Book